Guillaume Gaullier
@Guillawme@fediscience.org
Researcher from 🇫🇷 doing #cryoEM ❄️🔬 at Uppsala University 🇸🇪 Working on cyanobacterial carboxysomes 🦠, still interested in nucleosome 🧬🙃🧬 Mostly science posts and chatter in English, occasionally in French. Born at 356 ppm. #StructuralBiology #Biochemistry #Science #AcademicChatter #fedi22
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@Guillawme@fediscience.org
@GetzlerChem@mstdn.science As I was writing my first answer, I remembered this paper: https://doi.org/10.1038/s41467-023-41444-x
Controlled pH change in vitreous ice, with transient melting and rapid re-vitrification to allow a pH-dependent protein conformational change to occur. The very fact that this could work indicates that the initial vitrification did not alter the initial pH too much, at least with this buffer system.