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• Post #2309756

  Some questions for people into #cryoEM and #biochemistry All pKa values vary with temperature. This is especially apparent with Tris buffer: change of ~ +0.5 pH unit going from room temp to 4°C. Proton transfers in acid/base equilibria are very fast. Are they faster than vitrification? In other words, does a change of pKa (due to a rapid drop in temperature) of the buffer compound have the time to change the pH of the solution before it is fully vitrified? There are pH-sensitive fluorophores...

• Post #999410

  RE: https://wikis.world/@quarknova/116262006249339183 This is great news! I was in fact just coming to the realization that Wikipedia should be listed in my human.json file, so this resolution comes just at the right time.

• Post #714350

  RE: https://scholar.social/@khinsen/116249052896745512 This is an interesting experiment: bring back the old webring/blogroll concept based on trust between people, to help spot websites written by people in an ever deeper sea of generative AI content on the web. I just added it to my site (linked on my profile), and started building a list of the few sites I read regularly that are unambiguously crafted by humans. Happy to list other people's websites too, so long as they are written...

• Post #707036

  #AlphaFoldDB now has binary protein complexes! https://www.ebi.ac.uk/about/news/technology-and-innovation/first-complexes-alphafold-database/ #AlphaFold #AlphaFold2 #StructuralBiology