Post #2858524
2026-04-07 13:35 UTC
@lpryszcz@genomic.social @nanopore@genomic.social If it's stalling it's very curious why the k-mer is always the same. If it stalls at different sequences one would expect that the level would be different, right? I was kind of ignoring the problem so far, especially since in some of the runs we were abusing the RNA. But the latest run got 8% aligned reads, so it's a serious problem.
Replies (1)
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@lpryszcz@genomic.social 2026-04-07 13:42
@mzdravkov@mastodon.green i don't think it's sequence specific, those weird signals just get basecalled preferentially as AAG or other sequences, which is another issue. Have a look at raw signals